Journal:

Article Title: Application of the 5?-Nuclease PCR Assay in Evaluation and Development of Methods for Quantitative Detection of Campylobacter jejuni

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Figure Lengend Snippet: Amplification products from the C. jejuni-specific primers (A) and a universal 16S rRNA gene PCR primer pair (39) (B) for a set of Campylobacter strains. The samples were subjected to electrophoresis in 3% (A) and 2% (B) agarose gel at 100 V for 45 min. Ten microliters of the amplification product was loaded in each lane. Lanes: CSB, C. sputorum subsp. bubulis; CL, C. lari; CFF, C. fetus subsp. fetus; CC, C. concisus; AB, A. butzleri; AS, A. skirrowii; CJJ, C. jejuni subsp. jejuni; neg, negative control; mw, molecular weight marker.

Article Snippet: The following bacterial strains were used as negative controls (strains not assigned to any known culture collection are from private collections): C. coli (three strains, including the type strain [DSMZ 4689 T ]), C. concisus CCUG 13144 T (type strain; Culture Collection, University of Göteborg, Göteborg, Sweden), C. curvus CCUG 13146 T (type strain), C. fetus subsp. fetus , C. helveticus CCUG 30682 T (type strain), C. hyointestinalis subsp. hyointestinalis CCUG 14169 T (type strain), C. lari , C. rectus CCUG 20446B T (type strain), C. showae CCUG 30254 T (type strain), C. sputorum subsp. bubulis , C. sputorum subsp. sputorum , Actinobacter calcoaceticus , Arcobacter butzleri , A. cryaerophilus CCUG 17801 T (type strain), A. skirrowii CCUG 10374 T (type strain), Bacillus cereus , B. subtilis ATCC 6633 (American Type Culture Collection, Manassas, Va.), Bacteroides ureolyticus CCUG 7319 T (type strain), Brochothrix thermosphacta ATCC 11509 T (type strain), Carnobacterium divergens DSMZ 20623 T (type strain), C. gallinarum NCFB 2766 T (type strain; National Collection of Food Bacteria, Reading, England), Citrobacter freundii NCTC 9750 (type strain; National Collection of Type Cultures, Colindale, London, England), Clostridium perfringens ATCC 27324, Enterobacter aerogenes , E. agglomerans NCTC 9381 (type strain), E. cloacae NCTC 10005 (type strain), Enterococcus faecalis NCDO 2602 (type strain; National Collection of Dairy Organisms, Reading, England), Escherichia coli ATCC 8739, E. coli O157; H7, Flavobacterium odoratum ATCC 4651 (type strain), Flexispira rappini CCUG 23435, Helicobacter fennelliae CCUG 18820 T (type strain), H. pylori CCUG 15815 B T (type strain), Lactobacillus acidophilus (type strain), L. casei ATCC 393, L. curvatus DSMZ 20019 T (type strain), L. plantarum DSMZ 20174 T (type strain), L. sake subsp. sake DSMZ 20017 T (type strain), Listeria grayi , L. innocua DSMZ 20649 T (type strain), L. ivanovii , L. monocytogenes DSMZ 20600 T (type strain), L. seeligeri , L. welshimeri , Micrococcus luteus ATCC 272, Pseudomonas aeruginosa ATCC 15442, Salmonella enterica , S. enterica serovar Kentucky, Shigella sonnei ATCC 11060, Staphylococcus aureus ATCC 25923, Streptococcus pyogenes type 1 ATCC 12344 T (type strain), and Yersinia enterocolitica .

Techniques: Amplification, Electrophoresis, Agarose Gel Electrophoresis, Negative Control, Molecular Weight, Marker